The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf

Lu Ping

doi:doi:10.11648/j.sd.20160405.12

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The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf

Lu Ping

Published in Science Discovery (Volume 4, Issue 5)

Received: 25 August 2016 Accepted: Published: 25 August 2016

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Abstract

This study is aimed to clarify the mechanism of wolfberry fruit and leaf extract’s regulation of lipid metabolism, and to provide the necessary theoretical basis for the possible uses of wolfberry fruit extract as drugs improving lipid metabolism. We have investigated the effects of wolfberry extract on the proliferation and differentiation as well as lipid metabolism of preadipocytes on cell level, and have evaluated the influences of wolfberry extract on obese mice body weight, oral glucose tolerance and blood glucose. The comparison between the effects of different parts of wolfberry and the combination of experiments of cell and animal level are done for the first time. Results show that: neither wolfberry fruit nor wolfberry leaf has toxicity on 3T3-L1 preadipocytes; wolfberry extract can promote adipocyte differentiation and intracellular lipid accumulation, while wolfberry leaf does not have such effects; fruit extract can improve the C/EBPα, PPAR-γ gene expression levels and activate the transcriptional activity of PPAR-γ, while leaf does not have such effects; both wolfberry fruit and wolfberry leaf can reduce the blood glucose and improve the oral glucose tolerance of obese mice, and wolfberry fruit has a stronger effect, but in the meanwhile they also increase the mice’s body weight. Based on the results above, we can conclude that wolfberry fruit extract ingredients can regulate glucose and lipid metabolism to some extent. Thus, it has the potential to work as a kind of medicine to improve lipid metabolism and to cure type 2 diabetes.

Published in	Science Discovery (Volume 4, Issue 5)
DOI	10.11648/j.sd.20160405.12
Page(s)	275-279
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Wolfberry Extract, 3T3-L1 Preadipocytes, Adipocyte Differentiation, Glucose and Lipid Metabolism, PPAR-γ

References

[1]	张云霞,刘敦华.枸杞功能性成分研究进展及深加工发展趋势[J].食品与药品,2009,11(3):67-69.
[2]	黄琳娟,林颖,田庚元,等.枸杞子中免疫活性成分的分离、纯化及物理化学性质的研究[J].药学学报,1998,33(7):512-516.
[3]	杨东辉,王积福,魏璐雪.枸杞子浸膏甜菜碱的含量测定[J].中国中药杂志,1997,22(10):608-610.
[4]	彭光华,李忠,张声华.薄层色谱法分离鉴定枸杞子中的类胡萝卜素[J].营养学报,1998,20(1):76-78.
[5]	陈耀明.枸杞子浓缩汁维生素C含量测定法[J].医学争鸣,1994,15(4):304-305.
[6]	李春生,杜桂芝,赵全成,等.枸杞子化学成分的研究[J].中国中药杂志,1990,15(3):43-44.
[7]	黄琼,黄俊明,陈瑞仪,等.大豆、枸杞子、山楂复合提取物对小鼠化学性肝损伤的保护作用[J].营养学报,2003,25(2):208-211.
[8]	谢梅林,李松林.枸杞子提取物的抗衰老作用[J].中国野生植物资源,1996(2):9-11.
[9]	吴维明,冯前进,张立伟.枸杞子提取物对大鼠离体胸主动脉舒张作用的初步研究[J].山西中医学院学报,2007,8(2):18-19.
[10]	文建军.枸杞子提取物对各种常见细菌抑菌及抗炎作用实验研究[J].医药论坛杂志,2012,33(7):23-25.
[11]	陈葆荃,谢梅林,钱曾年,等.枸杞子提取物对小鼠免疫功能和果蝇寿命的影响[J].中国野生植物资源,1995(2):5-7.
[12]	李长江,张英锋,高大威.枸杞多糖分离纯化及降血糖效果的研究[J].燕山大学学报,2014(06):557-560.
[13]	丁园.枸杞多糖对2型糖尿病大鼠血糖、血脂的影响[J].辽宁医学院学报,2015,36(5):12-14.
[14]	齐宗韶,李淑芳,吴继平,等.枸杞子和枸杞叶化学成分的研究——第1报枸杞子和枸杞叶的营养成分[J].中国中药杂志,1986,11(3):41-43
[15]	何微,朱捷.枸杞叶中总黄酮及芦丁提取工艺的研究[J].当代化工,2015(11):2723-2725.
[16]	杨涓,魏智清,陈炜.枸杞叶降血糖作用的初步研究[J].四川中医,2009,27(4):47-49
[17]	黄欣,赵海龙,尹宏.青海枸杞叶提取物对低氧递增游泳训练小鼠运动能力的影响[J].辽宁中医药大学学报,2008,10(7):141-143.
[18]	Mcgarry J D. Banting Lecture 2001 Dysregulation of Fatty Acid Metabolism in the Etiology of Type 2 Diabetes [J]. Diabetes, 2002, 51(1):7-18.
[19]	李利平,付方明.PPARγ研究进展[J].国际内分泌代谢杂志,2003,23(1):29-32.
[20]	王丽,那威,王宇祥,等.鸡PPARγ基因的表达特性及其对脂肪细胞增殖分化的影响[J].遗传,2012,34(4):454-464.
[21]	Lehrke M, Lazar M A. The Many Faces of PPARγ [J]. Cell, 2005, 123(6):993-999.

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APA Style

Lu Ping. (2016). The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf. Science Discovery, 4(5), 275-279. https://doi.org/10.11648/j.sd.20160405.12

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ACS Style

Lu Ping. The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf. Sci. Discov. 2016, 4(5), 275-279. doi: 10.11648/j.sd.20160405.12

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AMA Style

Lu Ping. The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf. Sci Discov. 2016;4(5):275-279. doi: 10.11648/j.sd.20160405.12

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@article{10.11648/j.sd.20160405.12,
  author = {Lu Ping},
  title = {The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf},
  journal = {Science Discovery},
  volume = {4},
  number = {5},
  pages = {275-279},
  doi = {10.11648/j.sd.20160405.12},
  url = {https://doi.org/10.11648/j.sd.20160405.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160405.12},
  abstract = {This study is aimed to clarify the mechanism of wolfberry fruit and leaf extract’s regulation of lipid metabolism, and to provide the necessary theoretical basis for the possible uses of wolfberry fruit extract as drugs improving lipid metabolism. We have investigated the effects of wolfberry extract on the proliferation and differentiation as well as lipid metabolism of preadipocytes on cell level, and have evaluated the influences of wolfberry extract on obese mice body weight, oral glucose tolerance and blood glucose. The comparison between the effects of different parts of wolfberry and the combination of experiments of cell and animal level are done for the first time. Results show that: neither wolfberry fruit nor wolfberry leaf has toxicity on 3T3-L1 preadipocytes; wolfberry extract can promote adipocyte differentiation and intracellular lipid accumulation, while wolfberry leaf does not have such effects; fruit extract can improve the C/EBPα, PPAR-γ gene expression levels and activate the transcriptional activity of PPAR-γ, while leaf does not have such effects; both wolfberry fruit and wolfberry leaf can reduce the blood glucose and improve the oral glucose tolerance of obese mice, and wolfberry fruit has a stronger effect, but in the meanwhile they also increase the mice’s body weight. Based on the results above, we can conclude that wolfberry fruit extract ingredients can regulate glucose and lipid metabolism to some extent. Thus, it has the potential to work as a kind of medicine to improve lipid metabolism and to cure type 2 diabetes.},
 year = {2016}
}

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TY  - JOUR
T1  - The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf
AU  - Lu Ping
Y1  - 2016/08/25
PY  - 2016
N1  - https://doi.org/10.11648/j.sd.20160405.12
DO  - 10.11648/j.sd.20160405.12
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 275
EP  - 279
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20160405.12
AB  - This study is aimed to clarify the mechanism of wolfberry fruit and leaf extract’s regulation of lipid metabolism, and to provide the necessary theoretical basis for the possible uses of wolfberry fruit extract as drugs improving lipid metabolism. We have investigated the effects of wolfberry extract on the proliferation and differentiation as well as lipid metabolism of preadipocytes on cell level, and have evaluated the influences of wolfberry extract on obese mice body weight, oral glucose tolerance and blood glucose. The comparison between the effects of different parts of wolfberry and the combination of experiments of cell and animal level are done for the first time. Results show that: neither wolfberry fruit nor wolfberry leaf has toxicity on 3T3-L1 preadipocytes; wolfberry extract can promote adipocyte differentiation and intracellular lipid accumulation, while wolfberry leaf does not have such effects; fruit extract can improve the C/EBPα, PPAR-γ gene expression levels and activate the transcriptional activity of PPAR-γ, while leaf does not have such effects; both wolfberry fruit and wolfberry leaf can reduce the blood glucose and improve the oral glucose tolerance of obese mice, and wolfberry fruit has a stronger effect, but in the meanwhile they also increase the mice’s body weight. Based on the results above, we can conclude that wolfberry fruit extract ingredients can regulate glucose and lipid metabolism to some extent. Thus, it has the potential to work as a kind of medicine to improve lipid metabolism and to cure type 2 diabetes.
VL  - 4
IS  - 5
ER  -

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Author Information

Lu Ping

School of Life Science, Tsinghua University, Beijing, China

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Plain Text BibTeX RIS

APA Style

Lu Ping. (2016). The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf. Science Discovery, 4(5), 275-279. https://doi.org/10.11648/j.sd.20160405.12

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ACS Style

Lu Ping. The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf. Sci. Discov. 2016, 4(5), 275-279. doi: 10.11648/j.sd.20160405.12

Copy | Download

AMA Style

Lu Ping. The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf. Sci Discov. 2016;4(5):275-279. doi: 10.11648/j.sd.20160405.12

Copy | Download

@article{10.11648/j.sd.20160405.12,
  author = {Lu Ping},
  title = {The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf},
  journal = {Science Discovery},
  volume = {4},
  number = {5},
  pages = {275-279},
  doi = {10.11648/j.sd.20160405.12},
  url = {https://doi.org/10.11648/j.sd.20160405.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160405.12},
  abstract = {This study is aimed to clarify the mechanism of wolfberry fruit and leaf extract’s regulation of lipid metabolism, and to provide the necessary theoretical basis for the possible uses of wolfberry fruit extract as drugs improving lipid metabolism. We have investigated the effects of wolfberry extract on the proliferation and differentiation as well as lipid metabolism of preadipocytes on cell level, and have evaluated the influences of wolfberry extract on obese mice body weight, oral glucose tolerance and blood glucose. The comparison between the effects of different parts of wolfberry and the combination of experiments of cell and animal level are done for the first time. Results show that: neither wolfberry fruit nor wolfberry leaf has toxicity on 3T3-L1 preadipocytes; wolfberry extract can promote adipocyte differentiation and intracellular lipid accumulation, while wolfberry leaf does not have such effects; fruit extract can improve the C/EBPα, PPAR-γ gene expression levels and activate the transcriptional activity of PPAR-γ, while leaf does not have such effects; both wolfberry fruit and wolfberry leaf can reduce the blood glucose and improve the oral glucose tolerance of obese mice, and wolfberry fruit has a stronger effect, but in the meanwhile they also increase the mice’s body weight. Based on the results above, we can conclude that wolfberry fruit extract ingredients can regulate glucose and lipid metabolism to some extent. Thus, it has the potential to work as a kind of medicine to improve lipid metabolism and to cure type 2 diabetes.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - The Contrast and Evaluation of the Biological Effects on Glucose and Lipid Metabolism by the Extract from Wolfberry Fruit and Leaf
AU  - Lu Ping
Y1  - 2016/08/25
PY  - 2016
N1  - https://doi.org/10.11648/j.sd.20160405.12
DO  - 10.11648/j.sd.20160405.12
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 275
EP  - 279
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20160405.12
AB  - This study is aimed to clarify the mechanism of wolfberry fruit and leaf extract’s regulation of lipid metabolism, and to provide the necessary theoretical basis for the possible uses of wolfberry fruit extract as drugs improving lipid metabolism. We have investigated the effects of wolfberry extract on the proliferation and differentiation as well as lipid metabolism of preadipocytes on cell level, and have evaluated the influences of wolfberry extract on obese mice body weight, oral glucose tolerance and blood glucose. The comparison between the effects of different parts of wolfberry and the combination of experiments of cell and animal level are done for the first time. Results show that: neither wolfberry fruit nor wolfberry leaf has toxicity on 3T3-L1 preadipocytes; wolfberry extract can promote adipocyte differentiation and intracellular lipid accumulation, while wolfberry leaf does not have such effects; fruit extract can improve the C/EBPα, PPAR-γ gene expression levels and activate the transcriptional activity of PPAR-γ, while leaf does not have such effects; both wolfberry fruit and wolfberry leaf can reduce the blood glucose and improve the oral glucose tolerance of obese mice, and wolfberry fruit has a stronger effect, but in the meanwhile they also increase the mice’s body weight. Based on the results above, we can conclude that wolfberry fruit extract ingredients can regulate glucose and lipid metabolism to some extent. Thus, it has the potential to work as a kind of medicine to improve lipid metabolism and to cure type 2 diabetes.
VL  - 4
IS  - 5
ER  -

Copy | Download